POS0393 FIBRIN DEPOSITION IS AN ACTIVE TRIGGER OF CARTILAGE DEGENERATION IN RHEUMATOID ARTHRITIS
نویسندگان
چکیده
Background: Fibrin(ogen) maintains inflammation in various disorders but has never been linked to cartilage damage rheumatoid arthritis (RA) or other forms of inflammatory arthritis. Objectives: To investigate the role fibrin deposition on integrity Methods: Fibrin knee was analyzed by immunohistochemistry RA patients and murine adjuvant-induced (AIA). In chondrocytes, fibrinogen expression ( Fgα, Fgβ, Fgγ ) procoagulant activity were evaluated qRT-PCR turbidimetry respectively. Fibrin-induced catabolic genes assessed chondrocytes. Fibrin-mediated chondro-synovial adhesion (CSA) with subsequent tears studied co-cultures human autologous synoviocytes, AIA model, MRI. The link between calcification examined stained for calcific deposits vitro fibrinogen-stimulated Results: correlated severity explants wildtype (WT) mice, while deficient (Fg -/- mice protected. Accordingly, upregulated enzymes Adamts5 Mmp13 Secondly, CSA present fibrin-rich damaged WT not Fg mice. line, cultured explants, adhered exclusively fibrin-positive degraded areas. Gadolinium-enhanced MRI joints showed contrast-enhancement along surface controls. Finally, co-localized triggered chondrocyte mineralization inducing pro-calcification Anx5, Pit1, Pc1 cytokine (IL-6). Although at a much lesser extent, we observed similar fibrin-mediated mechanisms osteoarthritis (OA). Conclusion: directly impacts via induction catabolism, mechanical stress, calcification. Potentially, is key factor occurring as secondary consequence inflammation. Disclosure Interests: None declared
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ژورنال
عنوان ژورنال: Annals of the Rheumatic Diseases
سال: 2021
ISSN: ['1468-2060', '0003-4967']
DOI: https://doi.org/10.1136/annrheumdis-2021-eular.2521